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°í¸¶¹ÙÀÌ¿ÀÅØ(ÁÖ) - Mouse Tail Direct PCR Kit
» ÀÛ¼ºÀÚ : KSMCB » ÀÛ¼ºÀÏ : 2018-11-27 » Á¶È¸ : 148
» ÷ºÎÆÄÀÏ : ÷ºÎÆÄÀÏÀÌ ¾ø½À´Ï´Ù.
°í¸¶¹ÙÀÌ¿ÀÅØ(ÁÖ) - Mouse Tail Direct PCR Kit

Mouse tail·Î ºÎÅÍ DNA ÃßÃâ¾øÀÌ 10ºÐ¾È¿¡ ¹Ù·Î PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Â kit ÀÔ´Ï´Ù.

¡à DNA¸¦ »ÌÁö¾Ê°í mouse tail·Î ¹Ù·Î PCRÀ» ¼öÇàÇϹǷÎ, ½Ã°£ÀÌ ´ÜÃàµÇ¸ç °£ÆíÇÕ´Ï´Ù.

¡à Proteinase K (PK)¸¦ ó¸®ÇÒ Çʿ䰡 ¾ø¾î ºñ¿ë°ú ½ºÅÜÀ» ÁÙÀϼö ÀÖ½À´Ï´Ù.

¡à Overnight incubationÀº ±×¸¸! ´Ü 10ºÐ lysis °úÁ¤À» ÅëÇØ template¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.

¡à Hot Start TaqÀ» ÀÌ¿ëÇÏ¿© ºñƯÀÌ ¹ÝÀÀÀ» ÁÙÀ̰í PCR È¿À²À» ³ôÇû½À´Ï´Ù.

¡à Direct PCR buffer¸¦ mouse tail Á¶°Ç¿¡ ÃÖÀûÈ­½ÃÄÑ, ÁÁÀº °á°ú¸¦ º¸ÀåÇÕ´Ï´Ù.

¡à Á¶ÀÛÀ» ÃÖ¼ÒÈ­ÇÔÀ¸·Î½á ¿À¿°ÀÇ À§Çè°ú ¹ø°Å·Î¿òÀ» ÁÙ¿©ÁÝ´Ï´Ù.

¡à Transgenic mouse genotyping¿¡ Àû¿ëÇϸé È¿°úÀûÀÔ´Ï´Ù.

¡à Mouse ear·Îµµ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.


¡à
½ÇÇè°úÁ¤ÀÌ ´Ü¼øÇÕ´Ï´Ù.

1) Mouse tailÀ» 1-2mm Å©±â·Î ÀÚ¸¥´Ù.

2) ÇÔ²² Á¦°øµÇ´Â Lysis buffer¿¡ ³Ö°í 60°C¿¡¼­ 10ºÐ°£ incubation ÇÑ´Ù.

3) Vortex³ª spin down

4) »óÃþ¾× 1ul¸¦ ¶°¼­ ¹Ù·Î PCRÀ» ¼öÇàÇÑ´Ù.



Direct PCR from mouse tail tissue :

o 10mgÀÇ mouse tailÀ» lysis buffer·Î lysis¸¦ ¼öÇàÇÏ¿© °¢°¢ 2, 0.2, 0.04, 0.02ul ÀÇ ³óµµ·Î PCRÀ» ¼öÇàÇÏ¿´´Ù. PK 󸮾øÀÌ 60°C¿¡¼­ 10ºÐ incubation ¸¸À¸·Îµµ ±ú²ýÇÏ°Ô PCR ÁõÆøÀÌ ÀÌ·ç¾îÁüÀ» È®ÀÎÇÒ¼ö ÀÖ¾ú´Ù.

o Lane 1,3,5,7 : Direct PCR after lysis using Proteinase K at 56°C for 10 min and 95°C for 5 min

o Lane 2,4,6,8: Direct PCR without PK treatment. Incubation at 60°C for 10 min

- ¹®ÀÇó : °í¸¶¹ÙÀÌ¿ÀÅØ¢ß Tel:02-579-8787, Fax:02-578-7042 
              E-mail :
koma@komabiotech.co.kr, Hompage : http://www.komabiotech.co.kr

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