ICKSMCB 2019 / 2019 International Conference of the Korean Society for Molecular and Cellular Biology / September 17(Mon)-19(Wed), 2019 / COEX, Seoul, Korea


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GenScript USA Inc.: A Novel Endotoxin-free System for Recombinant Protein Expression

September 30 (Mon), 11:50-12:40, Rm. 307

Carlos Zhang, Ph.D.
Principal Project Manager, GenScript USA Inc.,

GenScript is the world leader in biotechnology reagent service industry that provides life science services and products to scientists in over 100 countries worldwide. Established in 2002 in New Jersey, United States, the company is the leading gene, peptide, protein and antibody research partner for fundamental life science research, translational biomedical research, and early stage pharmaceutical development.

Residual endotoxin in bacterial recombinant proteins has always been a big disadvantage in their application, and the remove of endotoxin causes additional cost burden. To address this, we have established a novel endotoxin-free recombinant protein expression system in the GRAS bacteria Bacillus subtilis. At the same time, the strong T7 promoter was engineered to construct a high-efficiency protein expression system in B. subtilis. The yields of tested antibody fragments were more than 5 mg/L and the endotoxin level were less than 2 EU/mg proteins without any endotoxin remove steps. Besides, this presentation will also introduce high-through-put gene-to-antibody service in mammalian system, which is capable of delivery of guaranteed high quality recombinant antibodies within 18 business days.