ICKSMCB 2019 / 2019 International Conference of the Korean Society for Molecular and Cellular Biology / September 17(Mon)-19(Wed), 2019 / COEX, Seoul, Korea

LUNCHEON SYMPOSIA

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GenScript USA Inc.: A Precise Technique for Construction of Diverse Variant Libraries

October 1 (Tue), 12:20-13:10, Rm. 401

An Introduction to Electronic Microscopy Methods and Correlative Microscopy for Life Science

Carlos Zhang, Ph.D.
Principal Project Manager, GenScript USA Inc., China

Variant libraries are widely applied for identification of critical residues of a protein or optimization of protein function, such as enhancing expression levels, protein stability and activity, or interaction with desired binding partners. Conventional methods for creating such libraries suffer from limited control over the codons introduced and variants can incur significant codon bias and even nonsense mutations. This results in a large library with over, underrepresented or incomplete variants, causing massive downstream screening efforts for researchers into capturing the entire variation of the library. We have developed a new method for creating variant libraries by our advanced semiconductor-based electrochemical oligonucleotide synthesis platform, which controls the mutated codons precisely without frequency bias. This novel technology builds a well-designed library containing all desired variants with unbiased distribution, minimizing the screening burden and saving valuable time and effort.