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¡°¼Õ´ë¸é Åå ÇÏ°í ÅÍÁú °Í¸¸ °°Àº ±×´ë, ºÀ¼±È¶ó ºÎ¸£¸®¡±. ÀÚ½ÄÀÌ ÁÁÀº °÷¿¡¼ Á¤ÂøÇÏ±æ ¹Ù¶ó´Â °Ç ½Ä¹°µµ ¸¶Âù°¡ÁöÀÎ µí, ½Ä¹°Àº Á¾ÀÚ¸¦ ¸Ö¸® º¸³»±â À§ÇØ ´Ù¾çÇÑ Àü·«À» °í¾ÈÇس»¾ú´Âµ¥, ºÀ¼±Èó·³ °Çµå¸®¸é Åö ÇÏ°í ¾¾¾Ñ ÁÖ¸Ó´Ï°¡ ÅÍÁ®¼ ¾¾¾ÑÀ» ¸Ö¸® ƨ°Ü º¸³»´Â ¹æ½Äµµ ±× Áß ÇϳªÀÌ´Ù. ÃÖ±Ù Æø¹ßÀû Á¾ÀÚ ºÐÃâ °úÁ¤ÀÇ ¸ÞÄ¿´ÏÁòÀÌ ¹àÇôÁ³´Âµ¥, ºñ´ëĪÀûÀÎ ¸®±×´ÑÀÇ ºÐÆ÷°¡ Á¾ÀÚ ºÐÃâ¿¡ ÇÊ¿äÇÑ ¿¡³ÊÁö¸¦ »ý¼ºÇÏ´Â Çٽɿ¡ Á¸ÀçÇÏ°í ÀÖ¾ú´Ù[1].
¼ºÀå°ú ÆØâÀ» ÅëÇØ Åº¼º¿¡³ÊÁö¸¦ ÀúÀåÇÏ°í, ÇÊ¿äÇÑ ¼ø°£¿¡ ÀúÀåµÈ ¿¡³ÊÁö¸¦ ºü¸£°Ô ¹æÃâÇÏ´Â °ÍÀÌ Æø¹ßÀûÀÎ Á¾ÀÚ ºÐÃâÀÇ ÇÙ½ÉÀε¥, ºÐÃâÀÌ ÀϾ´Â µ¿¾È ²¿Åõ¸®ÀÇ µÎ valves´Â ºü¸£°Ô ¹Ù±ùÂÊÀ¸·Î °¨±â¸é¼ ¿îµ¿¿¡³ÊÁö¸¦ Á¾ÀÚ¿¡ Àü´ÞÇØ ±×µéÀÇ ºÐÃâÀ» À¯µµÇÑ´Ù. endocarp b cellsÀÇ ÇÑÂÊ ¸é¿¡ °æø°ú °°Àº ¸ð¾çÀÇ ÇüÅ·ΠÃàÀûµÈ ¸®±×´ÑÀÌ ÀÌ·¯ÇÑ ¿¡³ÊÁöÀÇ È¿°úÀûÀÎ ÀúÀå°ú ¹æÃâÀ» °¡´ÉÇÏ°Ô ÇÏ´Â ÇÙ½É ¿äÀÎÀε¥, ºñ´ëĪÀûÀÎ ¸®±×´Ñ ±¸Á¶¿¡ ÀÇÇØ Á¶Á÷¿¡ µû¸¥ Â÷µî ¼öÃàÀÌ °¡´ÉÇØÁ® Àå·ÂÀ» »ý¼ºÇÏ°Ô µÇ°í, ÀÌ·¸°Ô »ý¼ºµÈ Àå·ÂÀÌ ÀÌÈÄ valveÀÇ ÄÚÀϸµ ÇüÅ·Πºü¸£°Ô ¿¡³ÊÁö¸¦ ¹æÃâÇÏ´Â °úÁ¤À» ÅëÇØ Á¾ÀÚ ºÐÃâÀÌ ÀϾ°Ô µÈ´Ù.
¼¼Æ÷ÀÇ Æ¯ÀÌÀû ±â´É ¼öÇàÀ» À§ÇÑ ¼¼Æ÷º®ÀÇ innovationÀº ÀÌ ¹ÛÀÇ ´Ù¾çÇÑ Á¶Á÷¿¡¼µµ º¸°íµÇ¾ú´Âµ¥, ²ÉÀÙÀ̳ª ÀÙÀ» º»Ã¼·ÎºÎÅÍ ºÐ¸®ÇÏ´Â °úÁ¤¿¡¼ Áß¿äÇÑ ¿ªÇÒÀ» ¼öÇàÇÏ´Â secession cellsÀÇ À°°¢Çü ±¸Á¶ÀÇ ¸®±×´Ñ[2], »Ñ¸®ÀÇ diffusion barrier¸¦ Çü¼ºÇÏ´Â ¸µ ±¸Á¶ÀÇ ¸®±×´ÑÀ¸·Î ÀÌ·ç¾îÁø, Casparian strip[3], ±×¸®°í »Ñ¸® ³»ÇǼ¼Æ÷¿¡¼ ¼öº£¸°ÀÇ Ä§Âø ¾ïÁ¦¸¦ ÅëÇØ Çü¼ºµÇ´Â passage cell[4,5]Àº ±× ´ëÇ¥ÀûÀÎ ¿¹µéÀÌ´Ù. ƯÈ÷³ª ÇغÎÇÐÀûÀÎ ±¸Á¶»ó ¾È°ú ¹ÛÀÇ ºÐ¸®¸¦ ´ã´çÇÏ´Â »óÇǼ¼Æ÷¿Í À¯»ç¼ºÀ» °®´Â »Ñ¸® ³»ÇǼ¼Æ÷¿¡¼ÀÇ ¼¼Æ÷º® ±¸Á¶´Â ¹°Áú ¼ö¼Û°ú Á÷Á¢ÀûÀ¸·Î ¿¬°áµÇ¾î ÀÖ¾î, ¼¼Æ÷º®ÀÇ ±¸Á¶Àû º¯È´Â ½Ä¹° ÀüüÀÇ ¿µ¾ç ¹× ¼öºÐ °ø±Þ¿¡ ¿µÇâÀ» ¹ÌÄ¡°Ô µÈ´Ù[6-8]. David Salt ±×·ì¿¡¼ ¾Ö±âÀå´ë ÀÙÀÇ nutrient elements¸¦ profilingÇÏ´Â ½ºÅ©¸®´×À» ÅëÇØ Ã£Àº mutants Áß ÇϳªÀÎ esb1 [9,10]Àº ÀÌÈÄ Casparian strip Çü¼º mutant ÀÓÀÌ ¹àÇôÁ³´Âµ¥[11], ÀÌ´Â »Ñ¸®ÀÇ ¼¼Æ÷º® Çü¼ºÀÌ ½Ä¹° Àü¹Ý¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Àß º¸¿©ÁØ ÀϷʶó ÇÏ°Ú´Ù.
ÀÌ·¸µí ¼¼Æ÷ÀÇ Æ¯¼ö¼ºÀ» ±¸ÇöÇϴµ¥ ¹ÐÁ¢ÇÏ°Ô °ü¿©ÇÏ°í ÀÖ´Â ¼¼Æ÷º®Àº ¾î¶² °úÁ¤À» ÅëÇØ Çü¼ºµÇ´Â °ÍÀϱî? ¿À·£ µ¿¾È °¡·ÁÁ®ÀÖ´ø ¼¼Æ÷º® ¸®¸ðµ¨¸µÀÇ ¸ÞÄ¿´ÏÁòÀÌ ÃÖ±Ù Casparian strip Çü¼º °úÁ¤À» ÅëÇØ ±× º£ÀÏÀ» ¹þ¾ú´Ù.
Casparian stripÀÇ ¸µ ±¸Á¶¿Í ÀÏÄ¡ÇÏ´Â À§Ä¡¿¡ Á¸ÀçÇÏ´Â ¼¼Æ÷¸· ´Ü¹éÁúÀÌ ¹ß°ßµÇ¾î CASPARIAN STRIP MEMBRANE DOMAIN PROTEINS (CASPs)À̶ó ¸í¸íµÇ¾ú´Ù [12]. ¼¼Æ÷¸·¿¡ Á¸ÀçÇÏ´Â ´Ù¸¥ ´Ü¹éÁúµéÀº Casparian strip À§Ä¡¿¡ ÇØ´çÇÏ´Â ¼¼Æ÷¸· ¿µ¿ª (CASPARIAN STRIP MEMBRANE DOMAIN, CSD)À¸·Î´Â À§Ä¡ÇÒ ¼ö ¾ø´Âµ¥ ¹ÝÇØ CASP1-5ÀÇ °æ¿ì´Â CSD¿¡ ƯÀÌÀûÀ¸·Î À§Ä¡ÇÏ¿´´Ù. ÈÄ¼Ó ¿¬±¸¸¦ ÅëÇØ ¸®±×´Ñ ÁßÇÕ°úÁ¤À» ¸Å°³ÇÏ´Â ´Ü¹éÁúµéÀÇ º£À̽º Ä·ÇÁ·Î½á ÀÛ¿ëÇÑ´Ù´Â °ÍÀÌ ¹àÇôÁ³´Âµ¥ [3], ¾Ö±âÀå´ë¿¡´Â ÀÌ¿Í À¯»çÇÑ CASP-like proteinsÀÌ 34°³ ´õ Á¸ÀçÇÏ¸é¼ cell type ƯÀÌÀû ¹ßÇöÆÐÅÏÀ» º¸ÀÌ°í ÀÖ¾î [13], À̵éÀÌ ¸®±×´Ñ »Ó ¾Æ´Ï¶ó ´Ù¾çÇÑ ¼¼Æ÷º®ÀÇ ¸®¸ðµ¨¸µ¿¡ °ü¿©ÇÒ °¡´É¼ºÀ» ½Ã»çÇØ ÁÖ°í ÀÖ´Ù. ÀÌ¿Í ´õºÒ¾î Casparian stripÀÇ positioningÀ» Á¶ÀýÇÏ´Â EXO70A1[14], receptor-like cytoplasmic kinaseÀÎ SCHENGEN1 [15], barrierÀÇ surveillance system ¿ªÇÒÀ» ÇÏ´Â receptor-like kinase SCHENGEN3/GASSHO1¿Í [8] ±×ÀÇ peptide ligandsÀÎ CASPARIAN STRIP INTEGRITY FACTORS (CIF1/2) [16]ÀÇ ¸ÞÄ¿´ÏÁòµµ ±Ô¸íµÇ¾úÀ¸¸ç, Endodermal identity¿¡ °ü¿©ÇÏ´Â Transcription factor MYB36¿Í signaling ³×Æ®¿öÅ© [17,18]µµ ±Ô¸íµÇ¾ú´Ù. ÀÌ·¯ÇÑ ÃÖ±ÙÀÇ ¹ß°ßµéÀº ½Ä¹° ¼¼Æ÷°¡ ¾ó¸¶³ª Á¤±³ÇÏ°Ô ¼¼Æ÷º®À» ¼¼°øÇÏ°í Àִ°¡ÀÇ ÇÑ ´Ü¸éÀ» µå·¯³»ÁÖ°í ÀÖ´Ù.
¼¼Æ÷ÀÇ °³º°Àû Ư¼ºÀ» ±¸ÇöÇϱâ À§ÇÑ ¼¼Æ÷º®ÀÇ ´Ù¾çÇÑ È°¿ë, ±×¸®°í À̸¦ À§ÇÑ Á¤±³ÇÑ ½Ä¹°¼¼Æ÷ÀÇ ¼¼°øÀÛ¾÷Àº ¼¼Æ÷º® ±¸Á¶°¡ ¼¼Æ÷ÀÇ Áö¹® ¿ªÇÒÀ» ÇÒ ¼öµµ ÀÖ´Â °Ô ¾Æ´Ò±î ÇÏ´Â »ó»óÀ» ºÒ·¯ÀÏÀ¸Å²´Ù. 20¿©³âÀü Brownlee ±×·ìÀº ÇØÁ¶·ù (Fucus spiralis)ÀÇ ¹è ¹ß»ý ¿¬±¸¸¦ ÅëÇØ ¼¼Æ÷º®ÀÌ Áö¹® ±× ÀÌ»óÀ¸·Î ¼¼Æ÷ÀÇ identity¸¦ À¯ÁöÇÏ´Â µ¥¿¡ Á÷Á¢ÀûÀ¸·Î °ü¿©ÇÒ °¡´É¼ºÀ» ½Ã»çÇÑ ¹Ù ÀÖ´Ù [19]. ¼¼Æ÷º®ÀÌ Á¦°ÅµÈ protoplasts´Â Å»ºÐÈ °úÁ¤À¸·Î ³Ñ¾î°¡Áö¸¸, ¼¼Æ÷º®°úÀÇ contactÀ» À¯Áö½ÃŲ °æ¿ì´Â ÀÚ½ÅÀÇ ¿ø·¡ ¿î¸íÀ» °í¼öÇÒ »Ó ¾Æ´Ï¶ó, ´Ù¸¥ cell fateÀ» °®´Â ¼¼Æ÷º®°úÀÇ contactÀº ±×ÂÊ ¿î¸íÀ¸·ÎÀÇ ÀüȯÀ» À¯µµÇÑ´Ù´Â °á°ú¸¦ º¸¿©ÁÖ¾ú´Ù. ÀÌ·¯ÇÑ °á°ú´Â ¼¼Æ÷º®ÀÌ ¼¼Æ÷ÀÇ identity¸¦ Çü¼ºÇØ °¡´Âµ¥ ÀÖ¾î ¼öµ¿ÀûÀÎ °á°ú¹° ±× ÀÌ»óÀÇ ¿ªÇÒÀ» ¼öÇà ÇÒ °¡´É¼ºÀ» ½Ã»çÇØÁÖ°í ÀÖ´Ù.
¾Æ¹«·± ¹æºñ°¡ ¾ø´Â »óÅ¿¡¼ ÃßÀ§¸¦ ¸¸³´Ù¸é ü¿Â À¯Áö¸¦ À§ÇØ ¶Û ¼ö¹Û¿¡ ¾øÀ» °ÍÀÌ´Ù. ±×·¯³ª ÁÖº¯¿¡ È°¿ëÇÒ ¼ö ÀÖ´Â µµ±¸µéÀÌ ÀÖ°í ±× µµ±¸µéÀ» È°¿ëÇÒ ´É·ÂÀÌ ÀÖ´Ù¸é, ±× »óȲ¿¡ óÇÑ ³ª´Â ¶Ù´Â ´ë½Å ÃßÀ§¸¦ ¸·À» ±¸Á¶¹°À» ¼¼¿ì·Á ÇÒ °ÍÀÌ´Ù. ±×·¯ÇÑ À¯»çÇÑ »óȲ¿¡ óÇÑ ³»°¡ 100¸íÂë ÀÖ´Ù°í ÇÒ ¶§, ³»°¡ ÇÏ´Â È°µ¿¸¸À» µé¿©´Ù º¸´Â °ÍÀº ³»°¡ óÇÑ »óȲÀ» ÀÌÇØÇϴµ¥ Å« µµ¿òÀÌ µÇÁö ¾ÊÀ» °ÍÀÌ´Ù. 100¸íÀÇ ´Ù¸¥ ³ª´Â ¸ðµÎ ¹«¾ð°¡ ±¸Á¶¹°À» ¼¼¿ì°í ÀÖÀ» Å×´Ï ¸»ÀÌ´Ù. ³»°¡ ¼¼¿ì°í ÀÖ´Â ±¸Á¶¹°ÀÌ ´Ù¸®ÀÎÁö, °Ç¹°ÀÎÁö, žÀÎÁö°¡ ÇÔ²² ÆľÇÀÌ µÇ¾î¾ß óÇÑ »óȲ°ú ÇÏ´Â ÀÏÀÌ ÃÑüÀûÀ¸·Î ÀÌÇØµÉ °ÍÀÌ´Ù. Single cell ·¹º§¿¡¼ À¯Àüü profilingÀÌ °¡´ÉÇÏ°í, À¯ÀüÀÚ °¡À§¸¦ ÅëÇØ Á¤±³ÇÑ À¯ÀüÀÚ ÀçÁ¶ÇÕÀÌ °¡´ÉÇÑ Áö±Ý, ¹Ù·Î Áö±ÝÀÌ ÆÐ¼Ç»Ó ¾Æ´Ï¶ó °úÇп¡¼µµ ¡®º¹°í¡¯°¡ ÇÊ¿äÇÑ ½ÃÁ¡ÀÌ ¾Æ´Ò±î ÇÑ´Ù.
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