Àß ¾Ë·ÁÁø »ç¶÷ ´Ü¹éÁúÀÇ 0.8%´Â RNA¸¦ ¼ö½Ä(modify)ÇÒ ¼ö ÀÖ´Ù [1]. DNA³ª ´Ü¹éÁú°ú ¸¶Âù°¡Áö·Î RNAµµ ±âº» ÈÇÐ ±¸Á¶¿¡¼ Ãß°¡ÀûÀÎ º¯È¸¦ °ÞÀ¸¸é ¾ÈÁ¤¼º°ú ±¸Á¶, ´Ü¹éÁú °áÇÕ µî¿¡¼ Â÷ÀÌ°¡ »ý°Ü³´Ù [2, 3]. ¾Æµ¥³ë½ÅÀÇ N6¿¡ ¸ÞÆ¿±â°¡ ºÙ´Â 6-methyladenosine (m6A), À¯¸®µòÀÇ N1 ´ë½Å C5¿¡ ´çÀÌ °áÇÕÇÏ´Â pseudouridine (ψ), ¹éº» 2′ ÀÚ¸®ÀÇ ¼ö»êȱ⿡ ¸ÞÆ¿ÀÌ Ãß°¡µÇ´Â 2′-O-methylation (Nm)ÀÌ ´ëÇ¥ÀûÀÌ´Ù. ¼ö½ÄµÈ ¿°±â°¡ °¡Àå ÈçÇÑ °÷Àº tRNA´Ù. ¼¼Æ÷ ¾ÈÀÇ tRNA¿¡¼´Â 20-30%ÀÇ ¿°±â°¡ ψ¿Í dihydrouridineÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ ÇüÅ·Πº¯ÇÑ´Ù. rRNAµµ ¶ÇÇÑ 7-methylguanosine (m7G), 1-methyladenosine (m1A) µîÀ» Æ÷ÇÔÇÑ´Ù. mRNAµµ ³·Àº ºóµµÀÌÁö¸¸ ¼ö½ÄÀÌ Á¸ÀçÇÑ´Ù. m6A°¡ °¡Àå Àß ¾Ë·ÁÁ® Àִµ¥, mRNAÀÇ ¾ÈÁ¤¼º°ú ´Ü¹éÁú »ý»ê È¿À²¿¡ ¿µÇâÀ» ¹ÌÄ£´Ù [3]. Æ÷À¯µ¿¹°ÀÇ mRNA¿¡¼ ψ, m7G, m1AÀÌ ¾ó¸¶³ª ±â´ÉÀÌ ÀÖÀ»Áö´Â ¾ÆÁ÷ Á» ´õ ¿¬±¸°¡ ÇÊ¿äÇÏ´Ù [4].
Àü»çü ¼öÁØ¿¡¼ ¼ö½ÄÀ» Ž»öÇÏ·Á¸é, »ýÈÇÐÀû Àüó¸® ÈÄ RNA-SeqÀ¸·Î º¯È¸¦ ã´Â´Ù. ¼¼ºÎÀûÀ¸·Î´Â Àüó¸® ¹æ¹ý¿¡ µû¶ó ´Ù¾çÇÑ Á¢±Ù¹ýÀÌ ÀÖ´Ù. MeRIP-seq¿¡¼´Â RNA¸¦
¾àÇÏ°Ô ÆÄÆíÈÇÑ µÚ¿¡, ¼ö½Ä ¿°±â¿¡ ´ëÇÑ Ç×ü·Î RNA¸¦ Á¤Á¦Çؼ ºÐ¼®ÇÑ´Ù. ÈÇйÝÀÀÀ» ÀÌ¿ëÇؼ Ç¥ÁöÇϰųª Á¤Á¦Çϱ⵵ Çϴµ¥, ψ¿¡ ´ëÇؼ´Â
N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide (CMC)¸¦ ÀÌ¿ëÇؼ ¼±ÅÃÀûÀ¸·Î Ç¥ÁöÇؼ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù [5, 6]. Ç×ü³ª
´Ü¹éÁúÀ» °øÀ¯°áÇÕÀ¸·Î ºÙÀÎ µÚ ³²Àº ºÎºÐÀ» ÀÌ¿ëÇؼ °ËÃâÇϰųª [7, 8], ¼ö½ÄµÈ ¿°±â¸¦ ÀÚ¸£Áö ¸øÇÏ´Â ¼±ÅÃÀû RNAºÐÇØÈ¿¼Ò¸¦ ó¸®Çؼ ¼¿ºÐ¼® °á°ú¿¡¼ »ý¼ºµÇ´Â ÆÐÅÏÀ» ºñ±³ÇÏ¿©
¼ö½ÄµÈ ¿°±âÀÇ À§Ä¡¸¦ È®ÀÎÇÑ´Ù [9].
2¼¼´ë ¼¿ºÐ¼®±â¸¦ È°¿ëÇÏ´Â ¹æ¹ýµéÀº ¿©·¯°¡Áö ´Ù¸¥ ¹æ¹ýÀ¸·Î ¸¸µç Á¤º¸¸¦ ´ë·®À¸·Î Àдµ¥ À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù. ÇÏÁö¸¸, 2¼¼´ë ¼¿ºÐ¼®±â´Â cDNA·Î ¹Ýµå½Ã º¯È¯ÇØ¾ß Çϱâ
¶§¹®¿¡ ¿ªÀü»ç °úÁ¤¿¡¼ Á¤º¸°¡ ¼Õ½ÇµÇ¸ç, ªÀº ¸®µå ±æÀÌ·Î ÀÎÇÏ¿© ½ºÇöóÀ̽Ì, Æú¸®A ²¿¸® °°Àº RNA ºÐÀÚ »óÅ°¡ ¼Ò½ÇµÈ´Ù. À¯ÀüÀÚ ¼öÁØ¿¡¼ ¼ö½Ä ºóµµ ºÐ¼®À̳ª »ùÇà °£ ºñ±³¿¡¼
ºóµµ º¯È¸¦ ºÐ¼®ÇÒ ¼ö´Â ÀÖÁö¸¸, À¯ÀüÀÚ ¶Ç´Â isoform ³»¿¡¼ ºÐÀÚº°·Î ¹ß»ýÇÏ´Â Â÷À̸¦ °üÂûÇÏ´Â µ¥´Â È°¿ëÇϱ⠾î·Æ´Ù. ¼¼Æ÷ ³»¿¡¼ RNAÀÇ »ý¾Ö ÁÖ±â Áß ´Ù¾çÇÑ ´Ü°è¸¦
°ÅÄ¡°í ¿©·¯°¡Áö ´Ù¸¥ ´Ü¹éÁú°ú »óÈ£ÀÛ¿ëÇÏ°í ÀÖ´Â ºÐÀÚµéÀÌ ¸ðµÎ È¥ÇÕµÈ Åë°è¸¸ º¼ ¼ö ÀÖÀ» »ÓÀÌ´Ù.
´ë¾ÈÀ¸·Î ±ä ¸®µå¸¦ Á¦°øÇÏ¸é¼ ÁõÆøÇÏÁö ¾Ê°í ¼¼Æ÷ À¯·¡ ºÐÀÚ¸¦ ±×´ë·Î ºÐ¼®ÇÒ ¼ö ÀÖ´Â ´ÜºÐÀÚ ¼¿ºÐ¼®±â°¡ È°¿ëµÇ±â ½ÃÀÛÇß´Ù. PacBioÀÇ Single Molecule, Real-Time (SMRT) ¼¿ºÐ¼®±â¿Í Oxford Nanopore Technologies (ONT)ÀÇ ³ª³ëÆ÷¾î ¼¿ºÐ¼®±â°¡ ´ëÇ¥ÀûÀÌ´Ù [10]. PacBio´Â ±ä ¸®µå¸¦ È°¿ëÇÏ¿© ºÐÀÚ ´ÜÀ§ ºñ±³°¡ °¡´ÉÇÏÁö¸¸ RNA´Â cDNA·Î ÀüȯÀÌ °ÅÀÇ ÇʼöÀûÀ̹ǷΠRNA ¼ö½ÄÀ» °³º°ÀûÀ¸·Î ºÐ¼®Çϱâ´Â ¾î·Æ´Ù. ONTÀÇ ³ª³ëÆ÷¾î ¼¿ºÐ¼®±â´Â ¸®µå ´ÜÀ§ÀÇ ¼¿ Á¤È®µµ°¡ 93–95% Á¤µµ·Î »ó¿ëÈµÈ ¼¿ºÐ¼®±â Áß °¡Àå ³·´Ù. ÇÏÁö¸¸, 100 kbp°¡ ³Ñ´Â ´õ¿í ±ä ¸®µå¸¦ Á¦°øÇϸç, DNAÁßÇÕÈ¿¼Ò¸¦ »ç¿ëÇÏÁö ¾Ê¾Æ ¿Ó½¼-Å©¸¯ ¿°±â½ÖÀ¸·Î °ËÃâÇÒ ¼ö ¾ø´Â ÇÙ»ê ¿°±âµéÀÇ ÈÇÐÀû Á¶¼ºÀ» Àо ¼ö ÀÖ´Ù. ³ª³ëÆ÷¾î ¼¿ºÐ¼®±â¿¡¼´Â Àü±â°¡ ÅëÇÏÁö ¾Ê´Â ¸· »çÀÌ¿¡ 1 nm ³»¿ÜÀÇ ±¸¸ÛÀ» ³»°í ±× »çÀÌ·Î ÇÙ»êÀ» Åë°ú½ÃŲ´Ù (±×¸² 1). ÇÙ»êÀÌ À̵¿ÇÏ¸é¼ ¿°±âÀÇ Å©±â¿Í ÀüÀÚ ¹Ðµµ¿¡ µû¶ó ±¸¸ÛÀ» ÅëÇØ Åë°úÇÒ ¼ö ÀÖ´Â ÀÌ¿ÂÀÇ ¾çÀÌ ½Ã½Ã°¢°¢ ¹Ù²î°Ô µÇ°í, ±× Àü·ù¸¦ ÃøÁ¤ÇÏ¿© ¼¿À» ºÐ¼®ÇÒ ¼ö ÀÖ´Ù (±×¸² 2).
óÀ½À¸·Î ¿°±â ¼ö½Ä °ËÃâÀÇ °¡´É¼ºÀÌ º¸ÀÎ °ÍÀº ¸ÞÆ¿ÈµÈ CpG DNA¿´´Ù. ¸ÞÆ¿ÈµÈ »çÀÌƼµò(5mC)ÀÇ °æ¿ì¿¡´Â C¿Í´Â ²Ï ´Ù¸¥ ½ÅÈ£¸¦ ¸¸µé¾î³Â±â ¶§¹®¿¡, ¿ÀÈ÷·Á DNA¼¿ºÐ¼®¿¡¼´Â
Á¤È®µµ Çâ»ó¿¡ ¹æÇØ°¡ µÆ´Ù. °ð 5mC¿Í 5-hydroxymethyl-2′-deoxycytidine (5hmC)¿¡ ´ëÇÑ ¸ðµ¨ÀÌ Á¤¸³µÇ¸é¼ À¯Àüü DNAÀÇ ¸ÞÆ¿È Áöµµ¸¦
¹Ý¼öüº°·Î ºÐ¸®ÇÏ¿© ÀÛ¼ºÇÏ´Â °ÍÀÌ Àüº¸´Ù ÈξÀ ¿ëÀÌÇØÁ³´Ù [11, 12]. ±× ¿Ü¿¡µµ 6-methyl-2′-deoxyadenosine (6mA)¿¡ ´ëÇÑ ¸ðµ¨µµ ¹ßÇ¥µÇ°í
[13, 14], 5-bromo-2′-deoxyuridine (BrdU), 5-ethynyluridine (EU) µî ´ë»çÇ¥Áö¸¦ ÀÌ¿ëÇؼ DNA ÇÕ¼ºÀÌ ÀϾ´Â ºÎºÐÀ»
³ôÀº È¿À²·Î Á¤È®ÇÏ°Ô Á¶»çÇÏ´Â ¹æ¹ýÀÌ µîÀåÇß´Ù [15, 16].
RNA ¼ö½Ä °ËÃâÀº ÃʱâºÎÅÍ ¸¹Àº ±â´ë¸¦ ¸ðÀ¸¸ç Á¦Ç° °³¹ß ´Ü°è¿¡¼ºÎÅÍ °¡Àå Áß¿äÇÑ °ü½É»ç¿´´Ù. ONT¿¡¼´Â m6A¿Í m5C°¡ °¢°¢ A¿Í C¿Í´Â ´Ù¸¥ ½ÅÈ£¸¦ ¹ß»ýÇؼ º£À̽ºÄÝ
¿À·ù¸¦ ¹ß»ý½ÃÅ°´Â »ç·Ê¸¦ º¸°íÇß´Ù [17]. UCSC¿¡¼´Â ¼¼±ÕÀÇ 16S rRNA¿¡¼ ψ¿Í m7G À§Ä¡¸¦ ¸¶Âù°¡Áö·Î º£À̽ºÄÝ ¿À·ù·Î ã¾Æ¼ ´Ù¸¥ ½ÅÈ£°¡ ¹ß»ýÇÏ´Â °ÍÀ»
¹ß°ßÇß´Ù [18]. ±×¸®°í, 2019³â¿¡´Â ³ª³ëÆ÷¾î ¼¿ºÐ¼®±â·Î RNA¸¦ ¿¬±¸ÇÏ´Â ÇÐ°è ¿¬±¸½ÇµéÀÇ ÄÁ¼Ò½Ã¾ö¿¡¼ Àΰ£ lymphoblastoid cell lineÀÎ GM12878¿¡¼
Àü»çü ¼öÁØ¿¡¼ mRNA¿¡¼ Æú¸®(A), ½ºÇöóÀÌ½Ì µî°ú ÇÔ²² m6A¸¦ ºÐ¼®Çß´Ù [19].
³ª³ëÆ÷¾î ¼¿ºÐ¼®±â·Î RNA ¼ö½Ä À§Ä¡¸¦ È®ÀÎÇÏ´Â ¼ÒÇÁÆ®¿þ¾îµéÀº ¹Ì¼¼ÇÑ Â÷À̵µ ¹Î°¨ÇÏ°Ô °ËÃâÇÒ ¼ö ÀÖµµ·Ï Àü·ù ½ÅÈ£ ´Ü°è¿¡¼ ÁÖ·Î ºÐ¼®ÇÑ´Ù. Nanopolish¿Í Tombo´Â
°ËÃâµÈ ½ÅÈ£¸¦ ÂüÁ¶ ¼¿¿¡¼ ¿¹»óµÇ´Â ½ÅÈ£¿¡ Á¤·ÄÇÑ µÚ¿¡, ÂüÁ¶ ¼¿ÀÇ °¢ ¿°±â À§Ä¡¿¡¼ Åë°è¼öÄ¡¸¦ °è»êÇÏ´Â ¹æ¹ýÀ¸·Î ¼ö½Ä À§Ä¡¸¦ ã´Â´Ù (Ç¥ 1). ½ÅÈ£¸¦ ´ëÁ¶ÇÏ´Â ¹æ¹ýµéÀº
RNAÀÇ ´Ù¾çÇÑ ºÎºÐ°ú ¿¬°è ºÐ¼®ÇÏ´Â »õ·Î¿î ±âȸ¸¦ Á¦°øÇßÁö¸¸ ¿©ÀüÈ÷ È°¿ëÀÌ ½±Áö´Â ¾Ê´Ù. ÇÑ ¿°±â°¡ ¾Æ´Ï¶ó ÁÖº¯ÀÇ 5–9°³ Á¤µµÀÇ ¿°±â°¡ ÇѲ¨¹ø¿¡ ƯÁ¤ ½ÃÁ¡ÀÇ Àü·ù¿¡
¿µÇâÀ» ÁÖ¸ç, RNA°¡ ¿òÁ÷ÀÌ´Â ¼Óµµ°¡ Å©°Ô ¹Ù²î¾î¼, ±â¼úÀÇ ¹Î°¨µµ¿Í È°¿ëµµ¸¦ Å©°Ô Á¦ÇÑÇÑ´Ù [10]. ºÐÀÚ ´ÜÀ§¿¡¼ Åë°èÀû ºÒÈ®½Ç¼ºÀÌ ³ô¾Æ ¼ö½Ä ºóµµ°¡ ³ôÀº °÷ÀÌ ¾Æ´Ï¸é
À§¾ç¼º·üÀÌ ³ô´Ù. Á¤È®µµ¸¦ ³ôÀÌÀÚ¸é ¹Ýµå½Ã ¾ç¼º ´ëÁ¶±ºÀÌ µÉ Ç¥ÁØ ÂüÁ¶ ¸ðµ¨À̳ª À½¼º ´ëÁ¶±ºÀÌ µÉ ¼ö½ÄÀÌ ¾ø´Â »ùÇà µî, ºñ±³ÇÒ Â¦ÀÌ ¹Ýµå½Ã ÇÊ¿äÇÏ´Ù. ½ÅÈ£ ¼öÁØÀÇ °ËÃâ¹ýÀº
¾ÕÀ¸·Îµµ ¸¹Àº °³¼±ÀÌ ÇÊ¿äÇÒ Àü¸ÁÀÌ´Ù.
À̸§ | ÃøÁ¤Ä¡ | ºñ±³ ¹æ¹ý | ÂüÁ¶ |
---|---|---|---|
Nanopolish | Æò±Õ Àü·ù (À̺¥Æ®) | ´ë¾È ¸ðµ¨ ´ëÁ¶ | [11] |
Tombo | Æò±Õ Àü·ù (ÂüÁ¶ ¿°±â ±âÁØ) |
|
[12] |
EpiNano | º£À̽ºÄÝ ¿¡·¯À² |
|
[20] |
ELIGOS | º£À̽ºÄÝ ¿¡·¯À² | »ùÇà ºÐÆ÷ °£ ´ëÁ¶ | [21] |
NanoCompore | Æò±Õ Àü·ù+Áö¼Ó ½Ã°£ (ÂüÁ¶ ¿°±â ±âÁØ) | »ùÇà ºÐÆ÷ °£ ´ëÁ¶ | [22] |
Á» ´õ °£´ÜÇÑ ¹æ¹ýÀ¸·Î ½ÅÈ£ Â÷ÀÌ¿¡ ÀÇÇØ ¹ß»ýÇÏ´Â º£À̽ºÄÝ ¿¡·¯¸¸ »ç¿ëÇϱ⵵ ÇÑ´Ù. ƯÁ¤ À§Ä¡¿¡¼ ¹ß»ýÇÏ´Â ¿¡·¯À²À» À½¼º ´ëÁ¶±º°ú ºñ±³ÇÏ¿© Åë°èÀûÀ¸·Î ¶Ñ·ÇÇÏ°Ô ¿¡·¯À²ÀÌ »ó½ÂÇÑ ºÎºÐÀ»
ã´Â ¹æ½ÄÀÌ´Ù. Yeast¿Í Arabidopsis¿¡¼ °¢°¢ m6A ¼ö½ÄÀ» ´ã´çÇÏ´Â È¿¼Ò¸¦ Á¦°ÅÇÏ°í º£À̽ºÄÝ ¿¡·¯¸¦ ºñ±³ÇßÀ» ¶§ ¾çÂÊ¿¡¼ ¸ðµÎ ¶Ñ·ÇÇÏ°Ô Àß ¾Ë·ÁÁø
DRACH ¸ðƼÇÁ°¡ ³ªÅ¸³µ´Ù [20, 23]. ONTÀÇ 2020³â 4¿ù ¾÷µ¥ÀÌÆ®¿¡¼± RNA ¼¿ºÐ¼® Á¤È®µµÀÇ ÃÖºó°ªÀÌ 93%±îÁö »ó½ÂÇؼ º£À̽ºÄÝ ¿¡·¯¸¦ ÀÌ¿ëÇÒ ¶§ ¹Î°¨µµ°¡ ¸¹ÀÌ
Çâ»óµÆ´Ù. »ç¿ëÇϱ⠽¬¿î ¼ÒÇÁÆ®¿þ¾î·Î ELIGOS, EpiNano µîÀÌ ³ª¿Í ÀÖ¾î¼ »ùÇà ¦¸¸ °®ÃçÁö¸é µµÀÔÇØ º¼¸¸ÇÑ °¡Ä¡°¡ ÀÖ´Ù [20-22]. ±×·¸Áö¸¸, ¿¡·¯À²Àº º£À̽ºÄÝ
¼ÒÇÁÆ®¿þ¾î¿Í ¸ðµ¨, ¸ðÅÍ ´Ü¹éÁú, Æ÷¾î ´Ü¹éÁú µî ¼ö¸¹Àº ¿ä¼ÒµéÀÇ ¿µÇâÀ» ¹Þ±â ¶§¹®¿¡ ¿Ïº®ÇÏ°Ô °°Àº Á¶°ÇÀ¸·Î ó¸®µÈ »ùÇà ¦ÀÌ ¾Æ´Ï¸é ºñ±³°¡ ¾î·Æ°í, ½Ã¾à°ú ¼ÒÇÁÆ®¿þ¾îÀÇ º¯È¿¡
µû¶ó ¼º´ÉÀÌ Å©°Ô ¿À¸£³»¸± ¼ö ÀÖ´Ù. ¶ÇÇÑ ¸®µå ´ÜÀ§¿¡¼ ¿¡·¯°¡ °¡Áö´Â Á¤º¸ÀÇ ¾çÀÌ ÀÛ¾Æ ºÐÀÚ ´ÜÀ§ ºÐ¼®¿¡´Â ¶Ñ·ÇÇÑ ÇÑ°è°¡ ÀÖ´Ù.
ÀÚ¿¬ »óÅÂÀÇ RNA¸¦ ±×´ë·Î ºÐ¼®Çϸ鼵µ ¼ö½ÄÀ» °ËÃâÇÏ´Â °ÍÀÌ ³ª³ëÆ÷¾î ¼¿ºÐ¼®ÀÇ Æ¯º°ÇÑ Á¡ÀÌÁö¸¸, Àü·ù°¡ ¶Ñ·ÇÇÏ°Ô º¯ÇÏÁö ¾Ê¾Æ °ËÃâÀÌ ºÒ°¡´ÉÇÑ °æ¿ìµµ Á¸ÀçÇÑ´Ù. ƯÈ÷
mRNA¿¡¼ÀÇ ±â´ÉÀÌ ¸¹Àº »ç¶÷ÀÇ °ü½ÉÀ» ²ô´Â m6A³ª ´ë»çÇ¥Áö ¹°Áú·Î °¡Àå ¸¹ÀÌ ¾²ÀÌ´Â 4-thiouridine (s4U)ÀÇ °æ¿ì, ¸¹Àº ¼öÀÇ ¹®¸Æ¿¡¼ Ç¥ÁØ ¿°±â¿Í Å« Â÷ÀÌ ¾ø´Â
½ÅÈ£¸¦ ¸¸µç´Ù. ¿©±â¿¡ Àü·ù¿¡ ¿µÇâÀ» Á٠ǥÁö¸¦ Ãß°¡Çؼ ½ÅÈ£ Â÷À̸¦ ¶Ñ·ÇÇÏ°Ô ¸¸µé¾î¼ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù [24]. Ç¥Áö È¿À²ÀÌ ½Ã¾àÀÇ Á¢±Ù¼º¿¡ µû¶ó ´Ù¸¥ Ư¼ºÀ» È°¿ëÇÏ¿© ¼¼Æ÷
³»¿¡¼ ƯÁ¤ ´Ü¹éÁú°ú °¡±î¿î °÷¿¡ Á¸ÀçÇÏ´Â RNA¸¦ Ç¥ÁöÇϰųª RNAÀÇ 2Â÷ ±¸Á¶¿¡ µû¶ó ¿°±â½ÖÀ» ÀÌ·çÁö ¾Ê´Â ºÎºÐ¿¡ ³·Àº È¿À²·Î Ç¥ÁöÇؼ ¼¼Æ÷ ³» 2Â÷±¸Á¶¸¦ ±ä ¼¿ÀÇ ¹®¸ÆÀ»
À¯ÁöÇÑ Ã¤·Î ºÐ¼®ÇÏ´Â µ¥¿¡ È°¿ëÇÒ ¼ö ÀÖ´Ù [25].
DNA ¼ö½ÄÀº ¾ÈÁ¤ÀûÀÎ ÂüÁ¶ ¸ðµ¨µéÀÌ °³¹ßµÇ¸é¼ º£À̽ºÄÝ·¯¿¡¼ ¼ö½Ä ¿°±âµéÀÌ Ç¥ÁØ ¿°±âó·³ Æ÷ÇԵǴ ¹æÇâÀ¸·Î ¹ßÀüÇÏ°í ÀÖ´Ù [26, 27]. RNA ¼ö½Äµµ Àå±âÀûÀ¸·Î´Â Ç¥ÁØ º£À̽ºÄÝ·¯¿¡ Æ÷ÇԵǰÚÁö¸¸, ÂüÁ¶ ¸ðµ¨À» ¸¸µå´Â µ¥ µå´Â ÀÚ¿øÀº ÈξÀ ¸¹ÀÌ µê¿¡µµ ºÒ±¸ÇÏ°í DNA¸¸Å ¼ö¿ä°¡ ¸¹Áö ¾Ê±â ¶§¹®¿¡ ´çºÐ°£Àº Áö±Ý°ú °°Àº »óȲÀÌ Áö¼ÓµÉ °ÍÀÌ´Ù. ¼¿ºÐ¼®±â´Â ´ë¿ë·® DNA ¼¿ºÐ¼®ÀÇ ±¤´ëÇÑ »ê¾÷¼ö¿ä ´ö¿¡ ³ôÀº º¹Àâµµ¿Í ¾ÐµµÀûÀΠ󸮴ɷ¿¡ ºñÇØ ºñ±³Àû Àú·ÅÇÑ °¡°ÝÀ¸·Î ´ë·®ÀÇ »ýÈÇÐÀû ÃøÁ¤À» Á¦°øÇÏ°í ÀÖ´Ù. ³ª³ëÆ÷¾î ¼¿ºÐ¼®±â´Â ƯÈ÷ Á¤º¸°¡ dzºÎÇÑ Àü·ù ½ÅÈ£ÀÇ ÀáÀç·ÂÀÌ Å©±â ¶§¹®¿¡ ¾ÕÀ¸·Îµµ RNA ¼ö½Ä°ú RNA °áÇÕ, ´ë»ç µî¿¡¼ Àü¿¡ ¾ËÁö ¸øÇß´ø »ý¸íÇö»óµéÀ» ¹ß°ßÇÒ ¼ö ÀÖÀ» °ÍÀÌ´Ù.
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