ICKSMCB 2022 / 2022 International Conference of the Korean Society for Molecular and Cellular Biology / September 28 - 30, 2022 / ICC JEJU

Nobel Laureate Lecture

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Koma Biotech

September 29(Thu), 12:40-13:05(25min.), 1F Yeongju Hall A

Kwon Yoo Wook

KAI1 (CD82) is a key molecule to switch angiogenic and hematopoietic milieu to quiescent state

Kwon, Yoo-Wook, Ph.D.
Biomedical Research Institute, Seoul National University Hospital, Korea

Little is known about endogenous inhibitors of angiogenic growth factors. In this study, we identified a novel endogenous anti-angiogenic factor expressed in pericytes and clarified its underlying mechanism and clinical significance. A peptide derived from the large extracellular loop of KAI1 has been shown to have anti-angiogenic effects to block the progression of breast cancer and retinal neovascularization in vivo. KAI1 from PC is a novel molecular regulator that counterbalances the effect of angiogenic factors.
Recently, we showed the evidence that DARC mRNA expression varies depending on subsets of macrophages and the DARC protein was expressed in a single cell level without complex with any other cells. Furthermore, we demonstrated the evidence of DARC expression in monocytes and macrophages in a single cell level. Finally, we confirmed the function of DARC+ macrophages for LT-HSC quiescence in vivo. For these experiments, we used tamoxifen-inducible monocyte /macrophage-specific DARC knockout mouse. In this study, we confirmed that DARC was expressed in a unique subset of monocytes and macrophages. These very special and rare macrophages expressing α-SMA+/Cox2+ & DARC+ (CD11b+Ly6G-F4/80+) are visualized in a single cell level through ImageStream (a flow cytometry-based single cell imaging technique).